AQUIOS Tetra – Fast, Efficient, High Throughput Lymphocyte Subset Analysis

In the clinical management of immune deficiency diseases, accurately counting the absolute cell numbers of leukocyte subsets and measuring the percentage of individual subtypes in blood is critical. The AQUIOS CL Flow Cytometer System in combination with AQUIOS Tetra reagents provides a Single Platform Technology (SPT) for lymphocyte subset analysis and enumeration without the need for counting beads.

The AQUIOS Tetra system tests for simultaneous identification and enumeration of T, B, and NK lymphocytes in whole blood are proven for reliability and precision and ideally complement the AQUIOS CL system. AQUIOS Tetra-1 and AQUIOS Tetra-2+ are for In Vitro Diagnostic Use. AQUIOS CL with Tetra-1 has been accepted for the World Health Organization list of prequalified in vitro diagnostic products.

Flow cytometry_B23533_AQUIOS Tetra-1 - Section 9

The AQUIOS reagents use a barcode identity for tracking reagent type, lot number, container number, expiration dates, and remaining reagent levels. The reagent consumption is monitored by the system as the samples are processed. Barcode tracking eliminates the need for manual QC or reagent logs and generates a full, easily reviewable audit trail. Should QC fail, operator is alerted via text message or email.

The AQUIOS CL System streamlines operations by incorporating automated loading, sample preparation, reagent management, and barcode scanning as well as data analysis and bidirectional LIS connectivity in one compact platform.

Aquios Tetra Table


Advanced Gating Algorithm for AQUIOS Tetra-1

To increase the accuracy of the lymphocyte gate, Beckman Coulter employs a parameter called Electronic Volume (EV) as a measure of relative cell volume.The AQUIOS CL flow cytometer incorporates this parameter to gate lymphocytes using Electronic Volume versus Side Scatter in a dual parameter gate. This additional gating element, added to the repertoire of lymphocyte subset analysis gating strategy, improves the purity and recovery of total lymphocytes in a way that balances purity and accuracy with speed.

Flow-cytometry-Aquios-CL-example-data-1_1 - Section 9



Purity and recovery are important

in the calculation of percentages

and absolute counts of lymphocyte

subsets.  Learn more about our

gating strategy for Tetra reagents

FLOWC-HIV-aquios gating strategy - Section 9

Contact Us

Please keep me informed about Beckman Coulter webinars, products, goods, and services, including products, goods, or services from our related companies.

By submitting this form I confirm that I have reviewed and agree with the Privacy Policy and Terms of Use. I also understand my privacy choices as they pertain to my personal data as provided in the Privacy Policy under “Your Privacy Choices”.

HIV Advanced Disease Management Solutions

CARES Solution man pipetting in hood

HIV Advanced Disease Management Solutions

Monitoring CD4 lymphocyte counts is essential in providing critical information that impacts patient care. CD4 monitoring allows caregivers to know when the disease transforms and stage its progression so they can implement the most appropriate intervention.. 

AQUIOS CL Tetra and Aged Whole Blood samples​

The use of flow cytometric analysis of peripheral whole blood to enumerate lymphocyte subsets is commonly used to assess the immunological status of patients in a wide variety of clinical conditions.

Flow cytometry_B23533_AQUIOS Tetra-1 - Section 9

AQUIOS CL Tetra for CD4 testing

In the clinical management of immune deficiency diseases, accurately counting the absolute cell numbers of leukocyte subsets and measuring the percentage of individual subtypes in blood is critical.

Aquios CL_Accessories_Tubes_White_MedRes - Section 9

AQUIOS CL Flow Cytometer for CD4 Testing​

Sample preparation and data management usually are the major bottlenecks in routine flow cytometry. Even with a semi-automated process, you and your team may spend hundreds of hours per year for tasks that don't add to your laboratory's productivity