ViaKrome Fixable Viability Dyes are used for the discrimination by flow cytometry of live and dead mammalian cells based on their fluorescence intensity. Since ViaKrome Fixable Viability Dyes are covalently bound to cellular proteins, the cells can further be processed through intracellular (Fixation and Permeabilization) and/or extracellular staining procedures prior to flow cytometry analysis.
The ViaKrome 405 Fixable Viability Dye is a thiol reactive fluorescent dye that covalently binds free thiol present on cellular proteins at a neutral pH. Live cells, impermeable to the ViaKrome 405 Fixable Viability Dye, will be weakly stained by the covalent binding of proteins express on the cell surface while cells whose membranes have lost structural integrity, will be strongly stained by the covalent binding of intracellular and membrane expressed proteins. The reactive groups are stable in aqueous solutions, thus the ViaKrome 405 Fixable Viability Dye does not require DMSO for reconstitution.
NOT ALL PRODUCTS ARE AVAILABLE IN ALL COUNTRIES
PRODUCT AVAILABILITY AND REGULATORY STATUS DEPENDS ON COUNTRY REGISTRATION PER APPLICABLE REGULATIONS
The listed regulatory status for products correspond to one of the below:
IVD: In Vitro Diagnostic Products. These products are labeled "For In Vitro Diagnostic Use."
ASR: Analyte Specific Reagents. These reagents are labeled "Analyte Specific Reagents. Analytical and performance characteristics are not established."
CE: Products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). (Note: Devices may be CE marked to other directives than (98/79/EC)
RUO: Research Use Only. These products are labeled "For Research Use Only. Not for use in diagnostic procedures."
LUO: Laboratory Use Only. These products are labeled "For Laboratory Use Only."
No Regulatory Status: Non-Medical Device or non-regulated articles. Not for use in diagnostic or therapeutic procedures.