Lipid Nanoparticles

Lipid nanoparticles (LNPs) are vital in drug and vaccine delivery, especially highlighted by their role in COVID-19 mRNA vaccines. They protect mRNA, siRNA, and pDNA from degradation, renal clearance, and immune responses, ensuring therapeutic efficacy. The next generation of LNPs, including solid lipid nanoparticles and nanostructured lipid carriers, offer improved architectures and physical stability. Their ability to control drug delivery timing and location makes them essential for treating various diseases and advancing personalized medicine.

Innovative LNP Formulation and Analysis Solutions

The increasing demand for novel lipid nanoparticle formulations is driving the need for high-throughput drug development and screening workflows. Beckman Coulter Life Sciences offers comprehensive solutions for producing and purifying nucleic acids for LNP loading, LNP mixing, drug formulation, and analytical characterization.

Lipid nanoparticles (LNPs) are a promising drug delivery system composed of a mix of various lipids (e.g., helper, ionizable, PEGylated, targeting) and often cholesterol with an encapsulated cargo. We offer a workflow solution to optimize LNP formulations, utilizing automation and ultracentrifugation, as well as solutions of biophysical and function studies with analytical ultracentrifugation and flow cytometry.

Analytical Characterization

API Production

LNP Formulation

Downstream Process

Analytical Characterization

API Characterization
Characterization of encapsulation efficiency (e.g., mRNA copy number), size distributions, and detection of unloaded LNPs
Functional studies for (e.g., cell uptake, protein expression)

Optima AUC Analytical Ultracentrifuge

CytoFLEX S Flow Cytometer

CytoFLEX nano Flow Cytometer

Biomek i-Series Automated Workstation

Echo 650 Series Acoustic Liquid Handlers

API Production

Production and Purification of API (e.g., RNA, DNA, peptide, small molecules, etc.).

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LNP Formulation

Formulation LNP’s containing the API of interest can be automated to improve throughput and repeatability.

Biomek i-Series Automated Workstation

Echo 650 Series Acoustic Liquid Handlers

Downstream Process

Optimization, concentration, and filtration of LNP formulations.

Biomek i-Series Automated Workstation

Optima X Series Ultracentrifuges

Optima Max Series Benchtop Ultracentrifuges

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Our Solutions

With our expertise and advanced technology, we are equipped to support your research and development efforts efficiently and effectively.

Optimization and Purification of LNPs:

Utilizing the Optima XPN and density gradient purification, LNP samples can be optimized for functionality.

Quantification and Characterization of LNPs:

Utilizing the CytoFLEX Nano Flow Cytometer, we provide loaded/unloaded ratios and functional assays. The Optima AUC Ultracentrifuge allows for precise quantification of LNP loading states, hydrodynamic radius, and RNA copy number distribution.