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Terminal deoxynucleotidyl transferase (TdT) is a nuclear enzyme with a molecular weight of 58kDa. This molecule catalyses random addition of nucleotides on the 3’ OH termini of single-stranded DNA. TdT differs from other DNA polymerases because it does not require a template sequence for polymerization. TdT is mainly expressed during the maturation of the immune system in primary and secondary lymphoid organ cells: i.e. precursors of B lymphocytes in the bone marrow and precursors of T-lymphocytes in the thymus. The TdT expression is not found in myeloid, erythroid, or megakaryocytic precursors, or in other non lymphoid cell components in every tissue, including mesenchymal, epithelial, and germ cells..
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