DNA and RNA Isolation from FFPE Tissue

FormaPure XL Total

Extract DNA and RNA from the same formalin-fixed paraffin-embedded tissue sample using SPRI paramagnetic bead-based chemistry.

FormaPure XL reagent kits are available in multiple kit sizes and extraction types based on your application and throughput needs. For optimum convenience, you also receive knowledgeable support for reagents and automation methods from a single vendor.

  • Scalable, single-chemistry system compatible with manual and automated processing (including sample deparaffinization and lysis)
  • Simple workflow with minimal hands-on-time
  • Demonstrated flexibility to accommodate sample input variation
  • High performance for yield, integrity and purity has been shown
  • Performance demonstrated with a variety of sample and tissue types
  • Demonstrated performance with 7x 10 μM curls

Spotlight Icon RFQ

Request Quote
Spotlight Icon Technical Documents

Technical Documents
Spotlight Icon Sample Data

Performance Data
Spotlight Icon Testimonials

Request Sample Kit
Flow Reagents Icon

Selection Guide
Spotlight Icon Technology

Liquid Handlers
Spotlight Icon Training

Training
Service Normal

Support
Spotlight Icon FAQ

FAQs

FormaPure XL Total Genomic Reagent Kits

FormaPure XL Total Visual Workflow

Genomics FormaPure Total XL Workflow

Product Specifications: FormaPure XL Total

Application Uses Nucleic Acid Sample Prep, Total NA Extraction, Total NA Isolation, Total NA Sequencing
Format Liquid
Starting Sample Material FFPE (formalin fixed paraffin embedded) tissue.
Automated Available Yes
Item Specifications Referenced C35991

Related Materials and Documentation

Title Description
Agencourt FormaPure Reagent Kits
{A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}
Manual or Automated RNA, DNA or Total Nucleic Acid Extraction from FFPE
Total Nucleic Acid Isolation from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues using FormaPure XL Total on a KingFisher™ Duo Prime
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
This protocol demonstrates the FormaPure XL Total performance on the KingFisher Duo Prime purification system. Automating chemistry can reduce the risk of human error, hands-on time (HOT) and total turn-around time (TAT).
Formalin-fixed and non-paraffin-embedded tissue DNA extraction
{7F90D59A-2B0F-4263-9E46-EE6BAB559DDB}
Here we demonstrated a DNA extraction protocol from formalin-fixed and nonparaffin-embedded brain tissue using FormaPure DNA XL kit. In this protocol, protein-DNA cross-links are reversed using heat
FormaPure Total Quick Guide
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
Checklist instructions for using the FormaPure Total extraction and purification kit.
FormaPure RNA: Extended Protocol for RNA Isolation from FFPE Sample
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
This document provides instructions for using the FormaPure RNA extraction and purification kit.
FormaPure Total: Protocol for DNA and RNA Isolation from the Same FFPE Sample
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
This document outlines the instructions for using the FormaPure Total extraction and purification kit.
SPRI Methodology
{9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}
Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.
FormaPure Total
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
FormaPure Totalの日本語簡易版マニュアルです。使用前には正式なマニュアル（英文）を必ずご一読ください。
FormaPureXL Total
{B14306BD-BB46-4B27-BF3E-B11862085BC7}
FormaPureXL Totalの日本語簡易版マニュアルです。使用前には正式なマニュアル（英文）を必ずご一読ください。
Simultaneously DNA and RNA extraction from formalin-fixed paraffin embedded (FFPE) tissue
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
In this poster we present a streamlined NGS ready to use sample prep workflow for FFPE tissue using a product in current development, FormaPure XL Total* (Beckman Coulter Life Science). FormaPure XL Total uses SPRI (Solid Phase Reverse Immobilization) paramagnetic beads to isolate DNA and RNA simultaneously.
AMP 2019: Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
In this poster, presented at AMP 2019, we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples
{C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}
In this poster we present a comparison of matched FFPE and plasma samples to determine how many mutations are seen in both tissues. We also look at where the mutational mismatches appear in the chromosome.
201901_interview
{D62418F5-2B8B-4155-AC0E-3ABFBF3E7911}|{AF9E1A10-BC29-46AA-A2A1-05BB530B6198}
FormaPure XL Data Sheet
{279C6CA2-9797-4CFE-9CAA-5EAFAC29B60D}
FormaPure XL reagent kits represent a single chemistry system designed and optimized for use in next-generation sequencing (NGS) as well as other downstream applications including qPCR/ddPCR/PCR
Manual or Automated RNA, DNA or Total Nucleic Acid Extraction from FFPE Tissues
{279C6CA2-9797-4CFE-9CAA-5EAFAC29B60D}
FormaPure XL reagent kits represent a single-chemistry system with demonstrated capability for use in Next-Generation Sequencing (NGS) as well as other downstream applications including qPCR. Maximizing integrity, yield and purity from an FFPE sample is required to minimize the risk of losing important genetic information
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisher™ Sample Purification System
{442DF88C-CA7F-4B78-AF75-9D2B985534C0}
This application note demonstrates the use of FormaPure XL Total, a total nucleic acid extraction kit for FFPE samples, in conjunction with the KingFisher™ Duo Prime Sample Purification System as a potential solution that mitigates some of the challenges with FFPE workflows.

Technical Documents