DNA Isolation from Blood

GenFind V3

Isolation and purification of genomic DNA (gDNA) from whole blood using Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology.

  • Consistently and optimal purity and quality of gDNA isolates
  • No carryover of inhibitors from anticoagulants, for exceptional PCR-based applications and NGS

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GenFind V3 Genomic Reagent Kits



GenFind V3 Isolation Workflow

Genomics GenFind V3 Workflow

Product Features

Overview
GenFind V3 isolates genomic DNA (gDNA) from whole blood and serum.

High Quality gDNA
The GenFind V3 DNA isolation method purifies gDNA that is intact and suitable for downstream PCR-based assays.

No Inhibition from Anticoagulants
GenFind V3 efficiently removes common anticoagulant and contaminants from blood such as citrate, EDTA, and heparin.

Product Specifications

Application Uses DNA Isolation, DNA Extraction, DNA sequencing, Genomic DNA
Format Liquid
Starting Sample Material Blood
Automated Available Yes
Item Specifications Referenced A83078

Documentation and Related Materials

Title Description
DNA extraction from up to 2 mL blood using GenFind V3
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Here we present a highquality, research-ready DNA extraction method from large volume whole blood by using GenFind V3.
DNA extraction from Zeesan@ Saliva DNA sample collection kit using GenFind V3
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Saliva contains buccal epithelial cells and white blood cells which provide rich genetic data. Due to the easy access and non-invasive character, saliva is an alternative to blood collection. Here we present a high-quality, DNA extraction method from saliva using GenFind V3.
DNA Extraction from Filamentous Fungi using GenFind V3
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This protocol provides two other methods for lysis that are more automation friendly and we show that they produce results that are very similar to the industry standard of bead beating.
DNA Isolation from Bacteria using GenFind V3
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This protocol provides a method for extracting DNA from bacteria with data showing its effectiveness with Escherichia coli, Staphylococcus aureus, Salmonella, Corynebacterium and Mycobacterium, a difficult to work with bacteria
Whole Blood DNA Extraction from PAXgene Blood DNA Tube using GenFind V3
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Here we show that the GenFind V3 protocol can process whole blood directly from PAXgene™ Blood DNA Tubes with no centrifugation step necessary
DNA extraction FTA cards or filter paper blood spots using GenFind V3
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This method provides instruction for additional lysis buffer volumes and optimized lysis conditions for extraction of DNA
DNA extraction from Mouthwash samples using GenFind V3
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Mouthwash samples are taken in large volumes (usually 10 - 30 mL), and cells need to be spun down from the total volume of mouthwash before extraction can begin. This protocol incorporates those steps and optimizes the lysis for mouth cell samples
Extraction of DNA from Mucoid Bacteria using GenFind V3
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This protocol reduces viscosity due to the presence of exopolysaccharides and decreases the amount of polysaccharide in the final eluate.
DNA extraction from fresh/frozen tissue using GenFind V3
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This provides a solution that allows a user to use one kit to extract DNA from multiple sample types with small modifications in the workflow.
DNA extraction from cell culture using GenFind V3
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This protocol provides a method for extracting DNA from cultured cells (≤ 2 million cells). Data shown shows the efficiency of this procedure with 1.4 million Jurkart cells, an immortalized line of human T lymphocyte cells
GenFindV2クイックマニュアル
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GenFindV2の日本語簡易版マニュアルです。使用前には正式なマニュアル（英文）を必ずご一読ください。
GenFindV3クイックマニュアル
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GenFindV3の日本語簡易版マニュアルです。使用前には正式なマニュアル（英文）を必ずご一読ください。
Comparison between mutation profiles of paired whole blood and cfDNA samples
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Next-Gen Sequencing of cfDNA is coming into maturity as a non-invasive method to identify mutational profiles in many cancer types
Comparison between Mutation Profiles of Paired Whole Blood and cfDNA Samples
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Review our poster we presented at ASHG 2019 where we looked at the data associated with the comparison between mutation profiles of paired whole blood and cfDNA samples
Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation
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Here, we demonstrate a walk-away automated solution for GenFind V3 kit to purify up to 400 µL of whole blood using the Beckman Coulter Biomek i7 Hybrid Genomics Workstation.
GenFind V3: Automated genomic DNA extraction from large volume whole blood
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In this poster we present an automated, high-quality, research-ready DNA extraction method from large volume blood. GenFind V3 DNA isolation kit automated on the KingFisher Duo Prime system provides scalable and consistent system for gDNA extraction from 2 mL of whole blood.
GenFind V3 DataSheet
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The method can be run manually in a 2 mL tube format or 96-well format, or automated in 96-well format on variety of Beckman Coulter Biomek liquid handling workstations
SPRI Methodology
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Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.


Technical Documents