MoFlo Astrios Cell Sorter

Simplify your most complex sorting tasks with the Astrios EQ Series of high-speed flow cytometry sorters. The Astrios EQ and EQs deliver powerful sorting capability while reducing complexity. 

  • Available with up to 7 spatially separated lasers and 51 parameters
  • Six-way jet-in-air proficiency
  • Dual forward scatter (EQ) and single forward scatter (EQs) models
  • Features [patent] enhanced Forward Scatter (eFSC) technology
     

MoFlo Astrios EQ Cell Sorter Features

Simplify your most complex sorting tasks with the Astrios EQ Series of high-speed flow cytometry sorters. The Astrios EQ and EQs deliver powerful sorting capability while reducing complexity. 

  • Available with up to 7 spatially separated lasers and 51 parameters
  • Six-way jet-in-air proficiency
  • Dual forward scatter (EQ) and single forward scatter (EQs) models
  • Features [patent] enhanced Forward Scatter (eFSC) technology

Optics

  • 7 laser / 7 pinhole configurable system; 355 nm, 405 nm, 488 nm, 532 nm, 560 nm, 592 nm, 645 nm.
  • Single Multi-Laser Shaping Optic (MLSO) for fiber-coupled lasers
  • Flat-Top beam profile on fiber coupled lasers increases beam stability and alignment ease
  • 7 lasers aligned through two alignment stages for quick sorter set up

Performance

  • IntelliSort II: Beadless drop delay determination and monitoring
  • 6-way sorting with a variety of collection devices (temperature control for all sort outputs available with water bath option)
  • Reproducibly sort up to 1536-well plates (due to historically 
stable fluidics)
  • Only sorter capable of 70,000 sort decisions/second validated 
performance

Electronics and Software

  • Electronic capability to simultaneously detect 44 parameters

  • > 100,000 events/second acquisition rate validated performance
  • 1 billion events .fcs files with no parameter limit
  • Up to 2 FSC channels including user-selectable FSC masks
  • Optional additional SSC detection off each wavelength greater than or equal to 405 nm

Biosafety

  • Custom Baker SterilGARD BSL2 cabinet
  • Single turnkey aerosol evacuation direct from sorter sort chamber to HEPA enclosure
  • Quick release nozzle design for easy access to nozzle tip
  • CyClone plate arm with wiper seals back of sort chamber

Explore MoFlo Astrios EQ Models

MoFlo Astrios EQ Cell Sorter Specifications

Regulatory Status RUO
Signal Processing Log, height, area, width & log area for each parameter
Resolution Resolves 0.2 μm diameter particles in scatter from background
Detection Up to 7 spatially separated lasers across 44 parameters acquired simultaneously (51 total available) , Dual Forward Scatter
Flow Cell Jet-in-Air Nozzle: 70 μm, 100 μm
Sorting Performance Configurable with 355, 405, 488, 532, 561, 592, 640 nm lasers
Drop Dive Frequency Adjustable up to 200kHz
Operating System Windows® 7 Professional
Software Summit v62
Power Requirements USA 115 VAC; 50/60 Hz at 15 A, EU: 220 VAC; 50/60 Hz at 10, JP: 100 VAC; 50/60 Hz at 15 A
Humidity Restrictions 20%-80% (non-condensing)
Weight 567 kg(1250 lb)
Depth 77 cm(30.3 in)
Width 165 cm(65 in)
Height 129.5 cm(51 in)

Content and Resources

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Flow-cell-sorting-moflo-astrios-eq-application-note-simultaneous-sorting-micro-macro-samples The MoFlo Astrios EQ series provides solutions to investigate biological materials between 200 nm and 30 μm
Flow-cell-sorting-moflo-astrios-eq-application-note-high-purity-nano-and-large-phytoplankton Phytoplankton conversion of light on the upper limits of the ocean consists of half of the photosynthesis on the Earth
Flow-cytometers-moflo-astrios-poster-forward-scatter Authors: Dr. Carley D. Ross, Mike Morrell, Robin Morris, Blake McCarty, Alan Dean Ph.D. and Nicole Lewis - Beckman Coulter, Inc., Flow Cytometry Business Center, Fort Collins, CO, USA
Flow-cytometers-poster-moflo-astrioseq-forward-scatter Authors: Carley D. Ross Ph.D., and Robin Morris Beckman Coulter Life Sciences, Flow Cytometry Business Center - Fort Collins, CO
Flow-cytometers-moflo-astrios-eq-brochure-cell-sorts-specfications Resolution: Resolves minimum 0.2μm diameter particles in forward scatter from background

Technical Documents