Multicolor Flow Cytometry

Seven Tips for Achieving the Perfect Panel

These 7 tips can help you improve the quality of your flow cytometry data and avoid common multicolor flow cytometry pitfalls.

Multicolor Flow Cytometry Resources

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Title	Description
Kaluza 1.5 Flow Cytometry Analysis Software {F0AFC765-5CD0-4BCE-BABE-164C20187E2F}
How does PROService receive information from the instrument? {C761825C-5739-42D5-90E3-D058FB587ADC}	How does PROService receive information from the instrument?
What do I need to do to prepare my site for PROService installation? {C761825C-5739-42D5-90E3-D058FB587ADC}	What do I need to do to prepare my site for PROService installation by Beckman Coulter Life Sciences?
How does Beckman Coulter use PROService to help me? {C761825C-5739-42D5-90E3-D058FB587ADC}	PROService tools and capabilities are used together to help maximize your instrument uptime, enhance productivity and increase efficiency in your lab
Can PROService be added to other Beckman Coulter instruments in the laboratory? {C761825C-5739-42D5-90E3-D058FB587ADC}	You can add PROService to any qualifying instrument at any time
Will the PROService connection put my instruments at risk? {C761825C-5739-42D5-90E3-D058FB587ADC}	Will the PROService connection put my instruments at risk?
Who should be available when PROService is being installed? {C761825C-5739-42D5-90E3-D058FB587ADC}	Who should be available when PROService is being installed?
What if my network has a proxy server for Internet access? {C761825C-5739-42D5-90E3-D058FB587ADC}	What if my network has a proxy server for Internet access?
What is the cost for PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	What is the cost of PROService at Beckman Coulter Life Sciences?
Can PROService be used for non-Beckman Coulter analyzers in the laboratory? {C761825C-5739-42D5-90E3-D058FB587ADC}	Can PROService be used for non-Beckman Coulter analyzers in the laboratory?
Practical Flow Cytometry {3AB28F00-B7FC-47D9-80F2-C0E49A388F7B}	Author: HOWARD M. SHAPIRO \| Published 2013
Flow Cytometry First Principles {3AB28F00-B7FC-47D9-80F2-C0E49A388F7B}	Author: Alice Longobardi Givan \| The Herbert C. Englert Cell Analysis Laboratory of the Morris Cotton Cancer Center and Department of Physiology Dartmouth Medical School - Lebanon, New Hampshire
Protection Plan for your Workflows in Flow Cytometry {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Maintenance service plan for Beckman flow analyzers.
Flow Cytometry Training {9E29D7A5-B5EC-470A-A5C1-4EF9BFAE9959}	Real-world training programs to maximize the value of your flow cytometer investment.
Kaluza for Gallios Acquisition Software Sophisticated Flow Cytometry, Simplified. {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Kaluza for Gallios from Beckman Coulter employs cutting-edge technology to make high complexity flow cytometry easy for even the least experienced, while providing powerful tools for the most sophisticated user
Specification Sheet: CytoFLEX LX Series U-V-B-Y-R-I {B14306BD-BB46-4B27-BF3E-B11862085BC7}	Technical Specifications for the CytoFLEX LX UV-Violet-Blue-Yellow Green-Red-Infrared (U-V-B-Y-R-I) Series of flow cytometers
Specification Sheet: CytoFLEX S Series V-B-R-I {B14306BD-BB46-4B27-BF3E-B11862085BC7}	Technical Specifications for the CytoFLEX S Violet-Blue-Red-Infrared (V-B-R-I) Series of flow cytometers
Specification Sheet: CytoFLEX LX Series N-V-B-Y-R-I {B14306BD-BB46-4B27-BF3E-B11862085BC7}	Technical Specifications for the CytoFLEX LX Near UV-Violet-Blue-Yellow Green-Red-Infrared (N-V-B-Y-R-I) Series of flow cytometers
Specification Sheet: CytoFLEX S Series V-B-Y-R {B14306BD-BB46-4B27-BF3E-B11862085BC7}	Technical Specifications for the CytoFLEX S Violet-Blue-Yellow Green-Red (V-B-Y-R) Series of flow cytometers
Specification Sheet: CytoFLEX Series V-B-R {B14306BD-BB46-4B27-BF3E-B11862085BC7}	Technical Specifications for the CytoFLEX Violet-Blue-Red (V-B-R) Series of flow cytometers
Cytomics FC 500 Flow Cytometry System with MXP Software – Part of the Cell Lab Family {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	With a host of innovative new features, the multi-platform loading Cytomics FC 500 MPL Flow Cytometry System delivers unprecedented productivity for today’s busy labs
Flow Cytometry — Powerful Versatile Performance {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	Built on a platform designed with reliability and stability in mind, Gallios samples information at 40MHz and displays data on a 1,048,576 channel scale.
Cytomics FC 500 With CXP Software {A7DF4F56-8AF9-4BF1-88BE-766AB73FBC6B}	This booklet introduces the Cytomics FC 500 with CXP software.
What hardware is required for PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	What hardware is required for PROService by Beckman Coulter Life Sciences?
How is PROService developed and maintained? {C761825C-5739-42D5-90E3-D058FB587ADC}	How is PROService developed and maintained?
Can third parties access my connected instruments, Remote Application Processor (RAP) box or data transferred by the RAP box? {C761825C-5739-42D5-90E3-D058FB587ADC}	Can third parties access my connected instruments, Remote Application Processor (RAP) box or data transferred by the RAP box?
What protected patient health information (PHI) may be viewed using PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	What protected patient health information (PHI) may be viewed using PROService?
Where is my instrument data stored when using PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	Where is my instrument data stored with Beckman Coulter Life Sciences?
What is the PROService installation process? {C761825C-5739-42D5-90E3-D058FB587ADC}	For some instruments, a Beckman Coulter field service engineer will configure and install the Remote Application Processor (RAP) box to enroll your instrument(s) in PROService
How secure is PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	How secure is Beckman Coulter's PROService?
Who can access my connected instrument(s) and related data through PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	Who can access my connected instrument(s) and related data through PROService?
How does PROService work? {C761825C-5739-42D5-90E3-D058FB587ADC}	PROService operates via a secure data pipeline remotely connecting Beckman Coulter to our systems/instruments located in customer labs
What is PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	What is PROService?
How many instruments can be connected to a PROService RAP box? {C761825C-5739-42D5-90E3-D058FB587ADC}	Up to five instruments can be connected to one Remote Application processor (RAP) box
How is PROService remote connectivity established? {C761825C-5739-42D5-90E3-D058FB587ADC}	How is Beckman Coulter's PROService remote connectivity established?
Where does the PROService Remote Application Processor (RAP) box reside? {C761825C-5739-42D5-90E3-D058FB587ADC}	Where does the PROService Remote Application Processor (RAP) box reside?
Can I obtain sodium azide-free formulations? {C761825C-5739-42D5-90E3-D058FB587ADC}	Yes. Purified antibodies can be formulated without azide for use in conjugation and functional studies, as they can be stored frozen to minimize the risk of microbial contamination. Or conjugates can be formulated at high concentrations with low amounts of sodium azide. This protects the conjugates from microbial contamination during repeated use, and the azide is diluted out in routine applications—which enables conjugate use with live cells and in cell-sorting studies.
Will we receive a compensation kit with our order? {C761825C-5739-42D5-90E3-D058FB587ADC}
How can we save money by using this service? {C761825C-5739-42D5-90E3-D058FB587ADC}	<p>You'll see an instant savings by eliminating the labor costs you currently incur to create large quantities of the cocktails you need. For DURA Innovations products, you could also save a significant amount of money each year by reducing reagent waste. Kindly contact your local Beckman Coulter sales representative to calculate the potential wastage due to reagent and time.</p>
Can you assist us with titrations? {C761825C-5739-42D5-90E3-D058FB587ADC}	<p>No. Products contracted under this service are manufactured to your specifications. Your laboratory should validate the products as necessary to meet your specific needs.</p>
Can you manufacture ASR- and/or IVD-labelled products for us? {C761825C-5739-42D5-90E3-D058FB587ADC}	<p>No. The FDA does not currently permit distribution of antibody cocktails, which identify multiple analytes, under the ASR designation. We will take your proprietary cocktail and manufacture it to your specifications in an ISO 9001-compliant facility following good manufacturing practices. As part of this strictly confidential process, you&rsquo;ll receive your products labelled only with:</p> <ul> <li>A brief description</li> <li>Volume</li> <li>Unique part and lot numbers</li> <li>Any applicable warnings</li> </ul>
Can any lab use this contract service? {C761825C-5739-42D5-90E3-D058FB587ADC}	Yes, service can provide benefits for many types of organizations
Can we use this service even if we don't have a flow cytometer from Beckman Coulter Life Sciences? {C761825C-5739-42D5-90E3-D058FB587ADC}	<p>Absolutely.</p>
Will we get a sample of our product to evaluate before committing to manufacturing it in bulk? {C761825C-5739-42D5-90E3-D058FB587ADC}	<p>Yes. After you give us your panel formulation, we'll provide you with 25 tests that you can evaluate for up to 4 weeks. After your approval, we'll proceed with production of your entire order. We can provide you with more than 25 tests for a modest additional fee.</p>
Which Beckman Coulter Life Sciences instruments are compatible with PROService? {C761825C-5739-42D5-90E3-D058FB587ADC}	Which Beckman Coulter Life Sciences instruments are compatible with PROService?
How long does it usually take to get a custom order? {C761825C-5739-42D5-90E3-D058FB587ADC}	Time to delivery depends on the availability of the raw materials, but we can usually deliver single-color conjugates within 4 to 6 weeks, and more complex multicolor reagents within 9 weeks to 12 weeks. With advance notice, repeat orders can be delivered in 4 weeks.<br> <br> If antibodies required for an order are unavailable in either a purified state or as ascites fluid, delivery may take longer.
What's the ordering process for made-to-specification antibody cocktails? {C761825C-5739-42D5-90E3-D058FB587ADC}	<div> <p><img alt="Contract Manufacturing Services Ordering Process" src="//media.beckman.com/-/media/flow-cytometry/products/reagents/custom-reagent-services/resource-contract-manufacturing-services/contract-manufacturing-process-cycle-2017-05.jpg?h=480&w=500&la=en-au&hash=584B4EAAAFDA761391311965B73051855613BE7D" style="width: 500px; height: 480px;"></p> <p><small><em>Contract Manufacturing Services Ordering Process</em></small></p> </div>
Can I use this service if Beckman Coulter Life Sciences doesn't offer the antibodies I need? {C761825C-5739-42D5-90E3-D058FB587ADC}	Yes. You simply provide us with the antibodies and instructions. Please note that the volumes required for some formulated reagents from other vendors may preclude use in dry-down formats.
Can we establish a delivery schedule that meets the needs of our operation? {C761825C-5739-42D5-90E3-D058FB587ADC}	<p>Yes. After you've approved and received your first order, we'll work with you to create a suitable delivery schedule for recurring orders of the same product. We suggest quarterly shipments of liquid formats, but for DURA Innovations products - which require no refrigeration - we can ship quarterly, three times/year, semiannually or even annually, depending on your needs and requirements.</p>
Is there a performance difference between liquid reagents and dry DURA Innovations reagents? {C761825C-5739-42D5-90E3-D058FB587ADC}	No. The proprietary DURA Innovations process yields a dry, stable product that delivers the same performance as a liquid product. Unlike liquid reagents, however, dry DURA Innovations-based products can be shipped and stored at room temperature.
Can my custom product be labeled as CE/ASR? {C761825C-5739-42D5-90E3-D058FB587ADC}	No. Because our custom products are manufactured using GMP* in ISO 9001-compliant facilities – but not under design control guidelines established by the US FDA or European directives – we cannot label them CE or ASR. All custom products you order will be labeled For Research Use Only.<br> <div> </div>
Will we get a pre-lot of our custom product to evaluate? {C761825C-5739-42D5-90E3-D058FB587ADC}	Yes. When you order a minimum of 500 tests, we’ll send you a pre-lot of 25 tests for evaluation.
What happens if we need to make changes to our product specifications? {C761825C-5739-42D5-90E3-D058FB587ADC}	<p>If you need to add/change a fluorochrome or antibody in the panel, or alter your delivery schedule, we'll need to amend your contract and possibly renegotiate pricing terms. Requests to increase the concentration of an existing marker in the panel will invariably require a contract amendment and an increase in price for the reconfigured product (after terms of any current contract have been met).</p>
Winner of First Global CARES HIV/AIDS Award Announced at IAS 2018 {78B55E4A-97D2-4630-88E1-2C5A1EEF617C}	<strong>MIAMI, US (July 18, 2018)</strong> ─ A 28-year-old African community youth worker Evah Namakula, has won the first, global CARES HIV/AIDS award, designed to recognize ordinary people who have shown ‘care, dedication and commitment’ in their communities as part of the fight against the disease

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