Stem Cells

Tools to improve workflow

Stem cell biology has emerged as one of the most significant fields in contemporary science, presenting the potential for a renewable source of replacement cells and tissues to treat a wide range of diseases.

Beckman Coulter offers instrumentation as well as a variety of stem cell-focused reagents for flow cytometry, cell screening, western blotting, ELISAs, cell differentiation and more.

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Stem Cell Resources

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Title	Description
Measuring Proliferation in Leukemia Cell Lines Using CFSE Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye which is cell permeable. It binds covalently to intracellular proteins. Fluorescence decreases over time as the dye is divided between daughter cells at each division. In this application note a detailed protocol for using this assay to assess cytotoxic effects of a pharmacologic agent is described.
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek® NXP Laboratory Automation Workstation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	To understand the changes that occur during differentiation of murine embryonic stem cells (mESCs)
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek® NXP Laboratory Automation Workstation {C00ACFAF-8661-4DF6-8BA5-A7A099A7BA9C}	Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter Inc, Indianapolis, IN
Monitoring Differentiation of Embryonic Stem Cells by Automated Flow Cytometry Sample Preparation on the Biomek NXP {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Amy Yoder, Michael Kowalski Ph.D., Li Liu Ph.D., Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter Inc., Indianapolis, IN \| Published 2011
Identifying Brain Tumor Initiating Cells Using Flow Cytometry Application Note {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Tumors represent a heterogenous population. Enumerating cells with stem cell like properties via co-expression of markers provides insight into the evolution of the tumor over time and throughout treatment. Detailed procedure for processing brain tissue for culture and flow cytometric analysis is provided.
Automated Sample Preparation Improves Throughput for Flow Cytometric Analysis of Mouse Stem Cell Populations {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: William L. Godfrey, Li Liu, Amy Yoder, and Laura Pajak \| Beckman Coulter, Inc., Miami, FL, USA and Indianapolis, IN, USA
MoFlo™ Viability and Function with High-Speed Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Sustained cell sorting at speeds in excess of 50,000 events per second is routine when using the MoFlo high-performance cell sorter
MoFlo™ Stem Cell Sorting {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Purified populations of functional stem cells are of great interest to the biomedical community, both in the understanding of stem cell biology and in clinical transplant settings
Automated Optimization of Murine Embryonic Stem Cell Differentiation into Cardiomyocytes {442DF88C-CA7F-4B78-AF75-9D2B985534C0}	Authors: Michael Kowalski Ph.D., Li Liu Ph.D., Amy Yoder, and Laura Pajak Ph.D. \| Life Science Automation, Beckman Coulter, Inc. Indianapolis, IN \| Published 2011

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