Error Prevention at Start Up / Cleaning / Worklist Generation on the AQUIOS CL System

The AQUIOS CL system employs several features that work to prevent errors during startup, cleaning, and worklist generation. These prevention methods can be broken down into the following:

  • Autocleaning
  • Test Requests to and from the LIS
  • Creating Worklists

Autocleaning
Autocleaning is a function integrated directly into the AQUIOS system. The AQUIOS CL flow cytometer continually monitors and detects potential clogs and/or air bubbles in the flow cell. Upon detection of a potential clog or bubble in the flow cell, the system aborts the sample run and activates an autocleaning cycle. Once this cycle is complete, the system will rerun the aborted sample. Autocleaning reduces the potential for low precision and erroneous results. In addition, autocleaning reduces the need for sample reruns.

Test Requests to and from the LIS
Traditional flow cytometers do not automatically transfer test requests to and from the LIS without the added expense of middleware. If the LIS connection is enabled on the AQUIOS CL flow cytometer, the system automatically retrieves the test request from the LIS. Users with Reviewer access are authorized to review and transmit samples to the LIS. Reviewer users are able to generate and perform test requests, and review and release results.

Not only does the built-in LIS ensure automatic transfer of test requests reducing transcription errors, but no middleware is required. Different user access levels ensure that operators can only work on areas that align with their level of experience with the system.

Creating Worklists
On a traditional flow cytometer, there is no direct connection from the worklist to sample preparation system, or sample preparation system to the analyzer. Traditional flow cytometers automatically send the results from the analyzer to the LIS and the test request from the middleware to the analyzer when using middleware. Potential errors may result because everything is not linked together. Refer to Figure 1 for a visual representation of an LIS connection using the FC 500 flow cytometer and TQ-Prep sample prep system. 8




FLOWC-HIV-LIS connection FC500 TQ Prep - Section 9



Conversely, the AQUIOS CL flow cytometer is one seamless system; no middleware is required. However, middleware can be used if desired. The system is able to send test requests to the analyzer and capture the information to automatically generate a worklist. Refer to Figure 2 for a visual representation of the LIS connection for the AQUIOS CL flow cytometer.

FLOWC-HIV-aquios-error prevention startup cleaning - Section 9

AQUIOS CL Flow Cytometer References
1. Evaluation of a novel automated volumetric flow cytometer for absolute CD4+ T lymphocyte quantitation
Gossez M, Malcus C, Demaret J, Frater J, Poitevin-Later F, Monneret G
Cytometry B Clin Cytom. 2017 Nov;92(6):456-464. doi: 10.1002/cyto.b.21360. Epub 2016 Feb 23.
https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.21360
2. Comparison of methods and TAT assessment: Volumetric AQUIOS CL and bead-based FACS CANTO II cytometers
Grossi V, Infantino M, Meacci F, Bellio E, Bellio V, Ciotta G, Priami F, Sarzi-Puttini P, Atzeni F, Li Gobbi F, Damiani A, Benucci M, Manfredi M
Cytometry B Clin Cytom. 2018 Jul;94(4):674-678. doi: 10.1002/cyto.b.21513. Epub 2017 Feb 15.
https://onlinelibrary.wiley.com/doi/abs/10.1002/cyto.b.21513
3. Performance verification of the new fully automated Aquios flow cytometer PanLeucogate (PLG) platform for CD4-T-lymphocyte enumeration in South Africa
Coetzee LM, Glencross DK
PLoS One. 2017; 12(11): e0187456. Published online 2017 Nov 3.  doi: 10.1371/journal.pone.0187456
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0187456
4. T lymphocyte immunophenotyping in bronchoalveolar lavage on AQUIOS CL cytometer.
Humeau C, Darrouzain F, Gouilleux-Gruart V
J Immunol Methods. 2018 Oct;461:91-99. doi: 10.1016/j.jim.2018.06.022. Epub 2018 Jul 3.
https://www.sciencedirect.com/science/article/pii/S0022175918300668?via=ihub
5. Analytical performance of an automated volumetric flow cytometer for quantitation of T, B and natural killer lymphocytes.
Degandt S, Peeters B, Jughmans S, Boeckx N, Bossuyt X.
Clin Chem Lab Med. 2018 Jul 26;56(8):1277-1288. doi: 10.1515/cclm-2017-0638.
https://www.degruyter.com/view/j/cclm.2018.56.issue-8/cclm-2017-0638/cclm-2017-0638.xml
6. Acceptable "Real-Life" Variability for Lymphocyte Counts by Flow Cytometry
Ticchioni M, Brouzes C, Durrieu F, Lambert C; Association Française de Cytométrie accreditation working group (AFC-AG).
Cytometry B Clin Cytom. 2018 Dec 7. doi: 10.1002/cyto.b.21751. [Epub ahead of print]
https://onlinelibrary.wiley.com/doi/abs/10.1002/cyto.b.21751
7. Advantages of the Use of AQUIOS CL Flow Cytometer in Evaluation of CD4 and CD8 in HIV Patients: CMCVAMC Experience
Patel S, Wolf B, Jhala D
American Journal of Clinical Pathology, Volume 150, Issue suppl_1, 21 September 2018, Pages S70, https://doi.org/10.1093/ajcp/aqy093.170
https://academic.oup.com/ajcp/article/150/suppl_1/S70/5103090









Efficiently manage your high

volume routine applications

with AQUIOS CL,  which enables

automation of your most

routine, repetitive tasks.

FLOWC_HIV CD4 London health Sciences AQUIOS - Section 7

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HIV Advanced Disease Management Solutions

HIV Advanced Disease Management Solutions

Monitoring CD4 lymphocyte counts is essential in providing critical information that impacts patient care. CD4 monitoring allows caregivers to know when the disease transforms and stage its progression so they can implement the most appropriate intervention.. 

Error Prevention and Sample Preparation with AQUIOS CL

The AQUIOS CL system employs several features that work to prevent errors during sample prep. These prevention methods can be broken down into the following:

Flow Cell Clogging Prevention

Reducing Tandem Dye Degradation/ Evaporation/ Condensation

Absolute Counting

Plus others

Error Prevention Overview with AQUIOS CL

Laboratory standards and regulations set forth by governmental bodies such as the Centers for Disease Control (CDC) help to define the procedures that laboratories follow

Error Prevention and Sample Preparation with AQUIOS CL ​

The AQUIOS CL system employs several features that work to prevent errors during sample prep. These prevention methods can be broken down into the following:

Flow Cell Clogging Prevention
Reducing Tandem Dye Degradation/ Evaporation/ Condensation
Absolute Counting
Plus others